DNA microarrays and gene expression by Pierre Baldi

Cover of: DNA microarrays and gene expression | Pierre Baldi

Published by Cambridge University Press in New York .

Written in English

Read online

Subjects:

  • DNA microarrays.,
  • Gene expression.

Edition Notes

Includes bibliographical references and index.

Book details

StatementPierre Baldi and G. Wesley Hatfield.
ContributionsHatfield, G. Wesley, 1940-
Classifications
LC ClassificationsQP624.5.D726 B353 2002
The Physical Object
Paginationxvi, 213 p. :
Number of Pages213
ID Numbers
Open LibraryOL22441131M
ISBN 100521800226
LC Control Number2001052862

Download DNA microarrays and gene expression

DNA MICROARRAYS AND GENE EXPRESSION From experiments to data analysis and modeling Massive data acquisition technologies, such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolu-tionizing biology and medicine.

Using the mRNA of a given cell, at a given. Overview. DNA microarray technology is a new and powerful means of analyzing genomes and characterizing patterns of gene expression. Its applications are widespread across the many fields of plant and animal biological and biomedical research.

Microarrays are not limited to the study of gene expression, but this remains the most common use of the technology and therefore is the only use of arrays discussed here. This book attempts to explain the underlying concepts and principles routinely used in 4/5(2).

Hybridization of fluorescently labeled cDNA preparations to DNA microarrays such as this provide a means for analyzing gene expression patterns on a genomic scale.

One example is the analysis of changes in gene expression as yeast cells convert from growth on a high-sugar medium to growth on by: DNA Microarrays: Gene Expression Applications Paperback – Aug by B.R. Jordan (Editor) out of 5 stars 2 ratings.

See all 2 formats and editions Hide other formats and editions. Price New from Used from /5(2). Get this from a library. DNA microarrays and gene expression.

[Pierre Baldi; G Wesley Hatfield] -- Massive data acquisition technologies--such as genome sequencing, high-throughput drug screening, and DNA arrays--are in the process of revolutionizing biology and. book that is self-contained and gives the reader a good sense of what is DNA Microarrays and Gene Expression: From Experiments to Data Analysis.

This method, gene expression profiling, is the major topic of this book. In its most simple sense, a DNA array is defined as an orderly arrangement of tens to hundreds of thousands of unique DNA molecules (probes) of known sequence.

There are two. DNA Microarrays and Gene Expression From Experiments to Data Analysis and Modeling. Get access. Using the mRNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell.

Such snapshots can be used to study fundamental biological phenomena Cited by: DNA MICROARRAYS GENE EXPRESSION APPLICATIONS Download Dna Microarrays Gene Expression Applications ebook PDF or Read Online books in PDF, EPUB, and Mobi Format.

Click Download or Read Online button to DNA MICROARRAYS GENE EXPRESSION APPLICATIONS book pdf for free now. DNA microarrays can be used to determine expression of multiple genes in a single reaction. 29 They utilize a multiplex spotted microarray technology, where thousands of oligonucleotide probes corresponding to human genes are spotted on a solid surface (gene chip) or on microscopic beads.

During the analysis, RNA isolated from a tumor sample is. The heady euphoria of the late nineties, promising that DNA microarrays would be ubiquitous tools for analysing gene expression and routinely used in every DNA microarrays and gene expression book biology laboratory, has been Author: S Russell.

1 Introduction to DNA Microarrays Chris Seidel Introduction Like strings of text in a book, the sequences that make up a strand of DNA have the gene expression DNA microarrays and gene expression book so that some number of RNA copies of a gene can Introduction j 3.

be synthesized. The RNA transcript has the same sequence as the gene with the. DNA Microarray. DNA microarrays are a well-established technology for measuring gene expression levels (potential to measure the expression level of thousands of genes within a particular mRNA sample) or to genotype multiple regions of a genome.

From: Principles of Translational Science in Medicine (Second Edition), Related terms: Protein. DNA microarrays, along with high-throughput RNAi screens, are the first fruits of the "functional genomics" movement available to C.

elegans researchers. By immobilizing miniaturized DNA probes that span the genome, DNA microarrays allow the rapid and economical—at least when compared with perform Northern blots—assessment of Cited by: analysis of microarray gene expression data Download analysis of microarray gene expression data or read online books in PDF, EPUB, Tuebl, and Mobi Format.

Click Download or Read Online button to get analysis of microarray gene expression data book now. This site is like a library, Use search box in the widget to get ebook that you want. Find many great new & used options and get the best deals for DNA Microarrays and Gene Expression: From Experiments to Data Analysis and Modeling by Pierre Baldi and G.

Wesley Hatfield (, Paperback) at the best online prices at eBay. Free shipping for many products. ISBN: OCLC Number: Description: xx, pages: illustrations (some color) ; 24 cm.

Contents: Microarrays in Gene Expression Studies --Background Biology --Genome, Genotype, and Gene Expression --Of Wild-Types and Other Alleles --Aspects of Underlying Biology and Physiochemistry --Polymerase Chain Reaction. Using microarrays, we can build a table of gene expression profiles that characterize the dynamic functioning of each gene in the genome by measuring gene transcription levels at different.

This chapter focuses on gene expression profiling, which is typically performed by the competitive hybridization of two samples, each labeled with a fluorescent dye such as cyanine 3-CTP or cyanine 5-CTP. We describe the steps, from RNA purification to data analysis, that are involved in obtaining data from DNA by: 7.

This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols.

It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and. Chapter 7describes gene expression analysis from cDNA microarrays and then chapter 8 covers the use of expression data in bioinformatics.

Chapter 9 describes the use of active microelectronic arrays for DNA hybridization analysis and the last chapter details the use of microarray technology inpharmacogenomics.

Book Description. DNA microarrays are in the process of revolutionizing biology and medicine. They can provide a snapshot of the level of gene expression in a cell and are therefore a powerful tool with which to study biological phenomena at the molecular level.

This book explores this powerful technology/5(2). Gene expression microarrays provide a snapshot of all the transcriptional activity in a biological sample. Unlike most traditional molecular biology tools, which generally allow the study of a single gene or a small set of genes, microarrays facilitate the discovery of totally novel and unexpected functional roles of by: The bacteriophage X was shown to transcribe RNA, which bound to DNA in a ribonuclease resistant complex.

Studies such as these established the mechanisms by which DNA is transcribed to RNA, and ultimately led to the development of hybridization-based assays, including microarrays.

The scale bar is μm. 9 Gene Expression: Microarray Data. to a gene (Figure 1A). The DNA in a spot may either be genomic DNA or short stretch of oligo-nucleotide strands that correspond to a gene. The spots are printed on to the glass slide by a robot or are synthesised by the process of photolithography.

Microarrays may be used to measure gene expression in many ways, but one of the. The use of miniaturized microarrays for gene expression profiling was first reported inand a complete eukaryotic genome (Saccharomyces cerevisiae) on a microarray was published in 4.

4 a) A DNA chip can be manufactured to contain hundreds of thousands of synthetic single-stranded DNA sequences. What is the technological differences between the DNA microarray and RNA microarray, specifically when gene expression is measured by each.

Wikipedia says "Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome.". DNA microarrays can be thought of as the massively parallel version of Northern blotting. While the Northern blotting technique is capable of analysing for each experiment the expression of one single gene in different conditions, microarrays allow the exploration of the expression levels of thousands of genes in several different conditions in.

Amplified differential gene expression microarrays (Zhijian J. Chen and Kenneth D. Tew) 4. DNA microarray detection and genotyping of human papillomavirus (TaeJeong Oh, SookKyung Woo, MyungSoon Kim, and Sungwhan An) 5.

Expression profiling of transcriptional start sites (Mutsumi Kanamori-Katayama, Shintaro Katayama, Harukazu Suzuki,Yoshihide Price: $ 1. Introduction: DNA Microarrays Ten Years Old, But No Old Hat 2.

cDNA Microarray Analysis And Its Role In Toxicology - A Case Study 3. Gene Expression Profiling In Plants Using cDNA Microarrays 4. Identification Of Gene Expression Patterns For. Introduction: DNA Microarrays – Ten Years Old, But No Old Hat 2. cDNA Microarray Analysis and Its Role in Toxicology - A Case Study 3.

Gene Expression Profiling in Plants Using cDNA Microarrays 4. Identification of Gene Expression Patterns for a. Here there is almost no overlap with Chapter 8 from DNA Microarrays: A Practical Approach on gene expression analysis. Chapter 9 describes some commercial products for the preparation and use of microarrays, and Chapter 10 presents an alternative labeling system (MICROMAX TM) developed by NEN to provide ultrahigh : Theodore E.

Mifflin. DNA Microarrays A Practical Approach Edited by Mark Schena Practical Approach Series. Written and edited by experts in the field, this book provides valuable insight into the remarkable advancement of DNA microarrays. It is essential for researchers investigating patterns of gene expression and useful for all those interested in the use of.

BASIC CONCEPTS OF MICROARRAYS. Microarrays can be distinguished based upon characteristics such as the nature of the probe, the solid-surface support used, and the specific method used for probe addressing and/or target detection ().Below, we review the methodologies of printed and in situ-synthesized microarrays, high-density bead arrays, and Cited by: Gene expression microarray (or DNA microarray) is a very powerful high-throughput tool capable of monitoring the expression of thousands of genes in an organism simultaneously.

The power of these tools has been applied to a range of applications, including discovering novel disease subtypes, developing new diagnostic tools, and identifying underlying mechanisms of disease. Massive data acquisition technologies, such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine.

Using the mRNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell/5(2). DNA Microarrays and Gene Expression: From Experiments to Data Analysis and Modeling | Pierre Baldi, G.

Wesley Hatfield, Wesley G. Hatfield | download |. Book Description. DNA microarrays are the modern, parallel version of classic molecular biology hybridization techniques allowing the exploration of thousands of sequences in a single run.

The technique permits the complete analysis of genetic material and the monitoring of expression changes occurring in a biological sample under various. A microarray is a multiplex is a two-dimensional array on a solid substrate (usually a glass slide or silicon thin-film cell) that assays (tests) large amounts of biological material using high-throughput screening miniaturized, multiplexed and parallel processing and detection methods.

The concept and methodology of microarrays was first introduced and illustrated in. A goal in expression analysis is to place all of the genes for an organism on a single chip and then use that microarray to monitor changes in gene expression in cells.

Already, there are chips with arrays representing all or many of the genes of the human and other species, such as rat, mouse, dog, monkey, honey bee, Arabidopis, Drosophila Cited by:

54784 views Thursday, November 12, 2020